Galectin—9蛋白过表达对卵巢癌细胞增殖、迁移及凋亡的影响

程亚玉+谭雪莹+黄飞

[摘要] 目的 探討半乳糖凝集素-9（Galectin-9）蛋白过表达对卵巢癌细胞增殖、迁移和凋亡的影响。 方法 将卵巢癌细胞SKOV-3分为转染pcDNA3.1-Galectin-9组、pcDNA3.1组和空白对照组，通过Western blot检测Galectin-9蛋白的表达，CCK-8检测Galectin-9蛋白对SKOV-3增殖能力的影响，划痕试验验证Galectin-9对SKOV-3迁移能力的作用，Annexin-V-FITC/PI双染法检测Galectin-9蛋白对SKOV-3凋亡的影响。 结果 Western blot结果显示，转染pcDNA3.1-Galectin-9组Galectin-9蛋白表达明显高于空白对照组和pcDNA3.1组，差异均有高度统计学意义（均P < 0.01）；CCK-8结果显示，转染pcDNA3.1-Galectin-9组增殖能力明显低于空白对照组和pcDNA3.1组，差异均有高度统计学意义（均P < 0.01），Galectin-9能显著抑制细胞的增殖能力，并且抑制细胞的增殖能力随着时间的延长而增强；细胞划痕试验结果显示，转染pcDNA3.1-Galectin-9组的愈合率明显高于pcDNA3.1组和空白对照组，差异均有高度统计学意义（均P < 0.01）；流式细胞试验结果显示，转染pcDNA3.1-Galectin-9组的凋亡率明显高于pcDNA3.1组和空白对照组，差异均有高度统计学意义（均P < 0.01）。 结论 Galectin-9的过表达能够抑制卵巢癌细胞株的增殖和迁移能力，并促进其凋亡。

[关键词] 半乳糖凝集素-9；卵巢癌；细胞增殖；细胞迁移；细胞凋亡

[中图分类号] R737.31 [文献标识码] A [文章编号] 1673-7210（2018）01（c）-0013-04

[Abstract] Objective To investigate the effects of overexpression of galectin-9 on the proliferation， migration and apoptosis of ovarian cancer cells. Methods The ovarian cancer cells SKOV-3 were divided into pcDNA3.1-galectin-9 transfection group， pcDNA3.1 group and blank control group， Western blot was used to detect the expression of galectin-9 protein， CCK-8 was used to detect the effects of galectin-9 protein for multiplication capacity of SKOV-3， scratch test was used to verify the effects of galectin-9 protein for transfer ability of SKOV-3， Annexin-V-FITC/PI double staining method was used to detect the effects of galectin-9 protein for apoptosis of SKOV-3. Results The results of Western blot showed that the expression of galectin-9 protein in pcDNA3.1-galectin-9 transfection group was significantly higher than those in blank control group and pcDNA3.1 group， the differences were all highly statistically significant （all P < 0.01）. The results of CCK-8 showed that the multiplication capacity of galectin-9 in pcDNA3.1-galectin-9 transfection group was significantly lower than those in blank control group and pcDNA3.1 group， the differences were all highly statistically significant （all P < 0.01）， galectin-9 could significantly inhibit the multiplication capacity of cells， and the capacity of inhibiting proliferation would increase with the extension of time. The results of cell scratch assay showed that the healing rate of pcDNA3.1-galectin-9 transfection group was significantly higher than that of pcDNA3.1 group and blank control group， the differences were all highly statistically significant （all P < 0.01）. The results of flow cytometry showed that the apoptosis rate of pcDNA3.1-galectin-9 transfection group was significantly higher than that of pcDNA3.1 group and blank control group， the differences were highly statistically significant （all P < 0.01）. Conclusion The overexpression of galectin-9 can inhibit the proliferation and migration of ovarian cancer cell lines and promote its apoptosis.endprint