Technology Research of Plantlets Rooting and Transplanting on Tissue Culture of Ilex centrochinensis

Shufang FAN

Jingchu U niversity of T echnology,Jingmen 448000,China

Technology Research of Plantlets Rooting and Transplanting on Tissue Culture of Ilex centrochinensis

Shufang FAN*

Jingchu U niversity of T echnology,Jingmen 448000,China

Plantlets of Ilex centrochinensis were used in its tissue culture to study the effect of auxin and gibberellin in vitro rooting,and the effect of various matrix in transplanting rooted plantlets and in rooting outside the tube.The highest frequency of rooting was obtained when growth regulator combination was IBA 0.2 mg/L+GA30.4 mg/L in vitro rooting,and when the ratio of sand and humus soil was 5 to 5 in rooting outside the tube.When the ratio of perlite and peat soil was 3 to 7,the highest survival of rooted plantlets in transplanting was obtained.This study may provide the basis for improving the factory system of tissue culture of Ilex centrochinensis.

Ilexcentrochinensis;Plantlets;Rooting;Domestication and transplanting

I lex centrochinensis belongs to Aquifoliaceae and Ilex,and also known as Ilex centrochinensis or Ilex micrococca Maxim.Because ofits unique morphological characteristics and health function,it was called Folium Ilicis Crenatae figuratively.Ilex centrochinensis has long life,lush foliage and peculiar leaf shape which the length of thorns was symmetrical. The leaves are evergreen and the flowers are yellow and the berries are red.Because of its highly ornamental value and various nutrient element, Ilex centrochinensis can be used for landscaping and used as medicine and tea.The germplasm resources of this plant is scarce.At present it was only found in Huanggang of Hubei province.The seeds had difficulty in propagation,and the plantlets were also difficult to rooting by cutting propagation.The studies of tissue culture of Ilex centrochinensis has not been reported.This study focused on the tissue culture rooting in vitro and rooting outside the tube,and transplanting of rooted plantlets,in order to find the best methods of rooting and transplanting.This study may provide the basis for improving the factory system oftissue culture of Ilex centrochinensis[1].

Materials and Methods

The determination of rooting medium

2 to 3 cm high and healthy testtube plantlets were taken to do rooting experiment.In this study,the basic medium was 1/2Nitch and the concentration gradientof growth regulator combination IBA was 0,0.2,0.4,0.8 mg/L.On the basis of 0.2 mg/L of IBA, different concentration of GA3that 0, 0.2,0.4 mg/L was added to analyze the role of GA on rooting.Each treatment group has 5 bottles and each bottle has 3 buds.Repeated 3 times[2-3].

Domestication and transplanting

The culture bottles were removed from culture room.One day later,the seals were loosened.Two days after, the seals were tore off.Four days later,the test-tube plantlets were cleaned and transplanted.The proportion of transplanting matrix on tissue culture of Ilex centrochinensis was showed in Table 1,and the matrix was sterilized in advance.Each treat-ment group has 5 test-tube plantlets. Repeated 3 times.

Table 1 The proportion oftransplantation matrix on Ilex centrochinensis

Rooting outside the tube

It was difficult to root outside the tube,and the rooting rate was not high.So 2 to 3cm high and healthy test-tube plantlets were taken to domestication and cuttage.The process of domestication was the same as transplanting,and the test-tube plantlets were treated by 0.5 mg/ml of NAA for 30 min.The matrix was sterilized in advance.Each treatmentgroup has 5 test-tube plantlets.Repeated 3 times[4]. cance testing.The results were showed in Table 2.

The results showed that,the rooting rate of each treatment was nearly when adding IBA to culture medium only.Considering the growth condition of root,the treatment group of 0.2 mg/L IBA was the best,and the treatment group of 0 mg/L IBA and 0.4 mg/L IBA was the second.After adding GA3extra,the rooting rate has a obvious upward trend,and the difference between each treatmentgroup was significant.The growth condition of root of the treatment group which added 30.4 mg/L GA was the best. The results were showed in Fig.1. Overall,the highestfrequency ofrooting was obtained when growth regulator combination was IBA 0.2 mg/L+ GA30.4 mg/L.

Table 2 The results ofrooting on Ilex centrochinensis

Results and Analysis

The determination of rooting medium

After 45 days of rooting culture, the results of different treatment group were analyzed by ANOVA and signifi-

The determination of transplanting matrix

After 30 days oftransplanting,the results of growth situation of rooted plantlets were shown in Table 3 and Fig.1.

The results showed that matrix proportion of pearlite and peat soilwas conducive to the survivaland growth of transplanted plantlets.When the matrix proportion of pearlite and peat soil was 3 to 7,bud ratio was highest,and the growth situation was also good. The matrix proportion of pearlite and

Table 3 The results oftransplanting on Ilex centrochinensis

humus soilwas more conducive to the survival and growth of plants.The results of Figure B of Fig.1 showed that the difference between rooted plantlets in difference matrix was notsignificant, and the plantlet grew faster after transplanting.Considering the later growth situation of plantlets,the matrix proportion ofpearlite and peatsoilwas 3 to 7 was better.Butitwas notsignificant with the treatment of sand and humus soil.In order to reduce the cost ofindustrialbreeding of Ilex centrochinensis,the matrix of sand and humus soil was better than the matrix of pearlite and peat soil in domestication and transplanting oftest-tube plantlets.

华中枸骨试管苗生根㈦驯化移栽技术的研究

范淑芳*（荆楚理工学院，湖北荆门448000）

以华中枸骨组培苗为材料研究了生长素和赤霉素对其试管内生根的影响，以及各种基质对生根苗移栽㈦试管外生根的影响。结果表明：华中枸骨试管内生根较好的生长调节剂配比为IBA 0.2 mg/L＋GA30.4 mg/L；生根苗移栽较好的基质配比为珍珠岩∶泥炭土= 3∶7；试管外生根较好的基质配比为河沙∶腐殖土=5∶5。该研究可为完善华中枸骨的组培工厂化体系提供依据。

华中枸骨;试管苗;生根;驯化移栽

范淑芳（1977-），女，湖北宜昌人，硕士，讲师，主要从事植物种质资源开发㈦利⒚方面的研究。*通讯作者，E-mail：fsf03@163.com。

2014-11-12

*Corresponding author.E-mail:fsf03@163.com

Received:November 12,2014 Accepted:December 30,2014

修回日期2014-12-30